Coding

Part:BBa_K1965023

Designed by: Katja Leben   Group: iGEM16_Slovenia   (2016-10-17)


Myc:nLuc:GS10:AP6

This construct consists of the N-terminus of the split luciferase that is connected to the AP6 coil. The construct also contains a Myc protein tag on the n-terminal side. The AP6 coil dimerizes to the P5 coil. When P5 contains cLuc and the two coils dimerize the two parts of the split luciferase come into close proximity, thus allowing for the reconstitution of the split luciferase and regaining its catalytic activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 103
    Illegal NgoMIV site found at 1451
    Illegal NgoMIV site found at 1472
    Illegal AgeI site found at 1175
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1357


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Categories
Parameters
None